KRONA plot of microbiomes of pooled samples from mouths, skin and Wastewater
datasetposted on 23.09.2020 by Ian Barry Goodhead
Datasets usually provide raw data for analysis. This raw data often comes in spreadsheet form, but can be any collection of data, on which analysis can be performed.
Unpublished data. To view the data, please download the file.
A KRONA plot displaying the relative abundance of bacteria identified in three pooled samples (Select the sample in the top left):
Pooled mouth samples from volunteers donating samples at Latitude Music Festival (mouth.fastq.out.cut).
Pooled skin samples from volunteers donating samples at Latitude Music Festival (skin.fastq.out.cut).
A pooled sample of sludge, mid-treatment, and treated wastewater from Bolton wastewater treatment plant (United Utilities); (wastewater.fastq.out.cut).
In each case, DNA from the samples was extracted using commercial DNA extraction kits (Qiagen) and the 16S gene amplified by Polymerase Chain Reaction (PCR) and sequenced using Oxford Nanopore reagents and MinION device. Further details can be found at: https://nanoporetech.com/applications/environmental-research